Acetoin Production from Agri-Waste Feedstocks

Acetoin (>98%) was purchased from Tokyo Chemical Industry Co., Ltd. (Shanghai, China). Acetonitrile (HPLC grade) was obtained from Sinopharm Chemical Reagent (Shanghai, China). Cassava powder and cottonseed meal were respectively obtained from Guangxi State Farms Mingyang Biochemical Group, Inc. (Nanning, China) and Jinan Oumi Biotechnology Co., Ltd. (Jinan, China). Thermostable α-amylase and glucoamylase obtained from Novozymes (Novozymes China Biotechnology Co., Ltd., Tianjin, China) were used for liquefaction and saccharification of raw starch as previously described [7 (link)]. The acid protease was purchased from Imperial Jade Biotechnology Co., Ltd. (Yinchuan, China) for protein hydrolysis [7 (link)].

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Diao M., Chen X., Li J., Shi Y., Yu B., Ma Z., Li J, & Xie N. (2023). Metabolic Engineering of Escherichia coli for High-Level Production of (R)-Acetoin from Low-Cost Raw Materials. Microorganisms, 11(1), 203.

Publication 2023

Acetoin Acetonitrile (HPLC grade) Thermostable α-amylase glucoamylase

Acetoin Acetonitrile Acid Amylase Cassava Glucoamylase Hplc Hydrolysis Powder Protease Protein Starch

Corresponding Organization : Guangxi Academy of Sciences

Other organizations : Guangxi University, Chinese Academy of Sciences, Institute of Microbiology

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Variable analysis

independent variables

Thermostable α-amylase
Glucoamylase

dependent variables

Liquefaction and saccharification of raw starch
Protein hydrolysis

control variables

Acetoin (>98%)
Acetonitrile (HPLC grade)
Cassava powder
Cottonseed meal
Acid protease

controls

Positive control: Thermostable α-amylase and glucoamylase for liquefaction and saccharification of raw starch, as previously described [7].
Negative control: Not explicitly mentioned.

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