Total RNA (8 μg) of each sample was processed to remove the ribosomal RNA using the Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA). Then, the rRNA-depleted RNA sample was used for the library construction using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, USA), as described (Zhang et al., 2018 (link)). Small RNA sequencing libraries of the two rabbit breeds were constructed using the Illumina TruSeq Small RNA Sample Preparation Kit v2, as described (Chen et al., 2019b (link)). Both stranded transcriptome sequencing and small RNA sequencing were performed on the HiSeq 2500 platform in NovoGene (Beijing, China).
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