Photographing Flaring Fish in Tanks

We photographed sexually mature fish inside a plastic tank using a Canon EOS RP with a Macro 100 mm lens (Macro Lens EF 100mm, Canon, Tokyo, Japan), as described (Kwon et al., 2022 (link)). All photos were taken inside a photo studio tent with white light emitting diodes. In each photo, a color card and ruler were visible, and a mirror was placed on the outside of the tank facing the fish to facilitate flaring, allowing for visualization of fins and consistency amongst photos. Before each imaging session, we calibrated the camera using the white balance (CT24-23-1424) on the 24ColorCard (CameraTrax).

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Palmiotti A., Lichak M.R., Shih P.Y, & Bendesky A. (2023). Genetic manipulation of betta fish. bioRxiv.

Publication Preprint 2023

EOS RP

Fish Lens Light

Corresponding Organization : Columbia University

Other organizations : Princeton University

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Variable analysis

independent variables

Presence of mirror on the outside of the tank facing the fish to facilitate flaring

dependent variables

Visualization of fins and consistency amongst photos

control variables

White light emitting diodes used in the photo studio tent
Color card and ruler visible in each photo
Camera calibration using white balance (CT24-23-1424) on the 24ColorCard (CameraTrax) before each imaging session

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