We photographed sexually mature fish inside a plastic tank using a Canon EOS RP with a Macro 100 mm lens (Macro Lens EF 100mm, Canon, Tokyo, Japan), as described (Kwon et al., 2022 (link)). All photos were taken inside a photo studio tent with white light emitting diodes. In each photo, a color card and ruler were visible, and a mirror was placed on the outside of the tank facing the fish to facilitate flaring, allowing for visualization of fins and consistency amongst photos. Before each imaging session, we calibrated the camera using the white balance (CT24-23-1424) on the 24ColorCard (CameraTrax).
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