Echocardiography and Blood Pressure Analysis in Mice

A Vevo 770 imaging system equipped with a 30 MHz high frequency scan head (Vevo770®, VisualSonics, Toronto, Canada) was used for echocardiography analysis according to the American Society for Echocardiography leading-edge method 16 . Mice were anesthetized with an isoflurane (2%) and O₂ (1.5L/min) mixture and positioned on a heated pad in a recumbent position. The end-diastolic interventricular septum thickness (IVSd), end-diastolic left ventricular (LV) posterior wall thickness (LVPWd), LV anterior wall thickness (LVAWd), LV end systolic diameter (LVSD), and end-systolic interventricular septum thickness (IVSs) were measured using M-mode. Ejection fraction (EF, %) and fractional shortening (FS, %) were calculated as described previously 17 (link). Systolic blood pressure (SBP) was measured noninvasively using a volume-pressure recording tail-cuff method on conscious, restrained mice using the CODA 6 system (Kent Scientific Corp, Torrington, USA).

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Zhao D., Wang W., Wang H., Peng H., Liu X., Guo W., Su G, & Zhao Z. (2017). PKD knockdown inhibits pressure overload-induced cardiac hypertrophy by promoting autophagy via AKT/mTOR pathway. International Journal of Biological Sciences, 13(3), 276-285.

Publication 2017

Vevo 770 CODA 6 system

Coda Conscious Diastolic Echocardiography Head Interventricular septum Isoflurane Left ventricular Mice Pressure Scan Systolic Systolic blood pressure Tail

Corresponding Organization : Shandong University

Other organizations : Affiliated Hospital of Shandong Academy of Medical Sciences, Shandong First Medical University, Shandong Chest Hospital, Wake Forest University, Qilu Hospital of Shandong University

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Variable analysis

independent variables

Isoflurane (2%) and O2 (1.5L/min) mixture

dependent variables

End-diastolic interventricular septum thickness (IVSd)
End-diastolic left ventricular (LV) posterior wall thickness (LVPWd)
LV anterior wall thickness (LVAWd)
LV end systolic diameter (LVSD)
End-systolic interventricular septum thickness (IVSs)
Ejection fraction (EF, %)
Fractional shortening (FS, %)
Systolic blood pressure (SBP)

control variables

Mice were anesthetized and positioned on a heated pad in a recumbent position
Echocardiography analysis was performed according to the American Society for Echocardiography leading-edge method

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