Immunohistochemical Analysis of CTHRC1 and Integrin β3 in Tumors

Tumors were collected to fixed and embedded to be made into paraffin sections. Then sections were incubate with primary antibodies against CTHRC1 (Abcam, London, UK) and integrin β3 (Abclonal, Wuhan, China) at 4 °C overnight which were followed by the incubation of the corresponding secondary antibodies and DAB chromogenic reaction and HE staining [38 (link)].
Membranous PSMA quantification was determined by modified H-Score (H-SCORE = ∑ (pi × i) = (percentage of weak intensity area ×1)+(percentage of moderate-intensity area ×2)+(percentage of strong intensity area ×3), to determine the overall percentage of mPSMA positivity across the entire stained tumor sample, yielding a range from 0 to 300 [39 ].

Free full text: Click here

Shen R., Cai X., Shen D., Zhang R., Zhang W., Zhang Y., Li Y., Wang A., Zeng Y., Zhu J., Liu Z, & Huang J.A. (2022). Long noncoding RNA LINC00518 contributes to proliferation and metastasis in lung adenocarcinoma via the miR-335-3p/CTHRC1 Axis. Cell Death Discovery, 8, 98.

Publication 2022

CTHRC1

Antibodies Chromogenic Integrin Membranous Paraffin Tumor Weak

Corresponding Organization :

Other organizations : Soochow University, First Affiliated Hospital of Soochow University

Top 5 similar protocols

Variable analysis

independent variables

Primary antibodies against CTHRC1 (Abcam, London, UK) and integrin β3 (Abclonal, Wuhan, China)

dependent variables

Membranous PSMA quantification determined by modified H-Score (H-SCORE = ∑ (pi × i) = (percentage of weak intensity area ×1)+(percentage of moderate-intensity area ×2)+(percentage of strong intensity area ×3), to determine the overall percentage of mPSMA positivity across the entire stained tumor sample, yielding a range from 0 to 300

control variables

Tumor sections were fixed and embedded in paraffin
Sections were incubated with primary antibodies and corresponding secondary antibodies
DAB chromogenic reaction and HE staining were performed

positive controls

No positive controls were explicitly mentioned

negative controls

No negative controls were explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!