Cholangiocyte isolation and IMCL transfection

Cholangiocytes were obtained by immunoaffinity separation (4 (link)). The in vitro studies were performed in vector or Sct stable-transfected IMCL (4 (link)). The IMCL control (stable transfected with the empty vector) or IMCL lacking Sct was established using SureSilencing short hairpin RNA (Super-Array, Frederick, MD) plasmid for mouse Sct containing a marker for neomycin resistance for the selection of stably transfected cells. The human hepatic stellate cells (HHSteC) were purchased from Sciencell (Carlsbad, CA).

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Wu N., Meng F., Invernizzi P., Bernuzzi F., Venter J., Standeford H., Onori P., Marzioni M., Alvaro D., Franchitto A., Gaudio E., Glaser S, & Alpini G. (2016). The secretin/secretin receptor axis modulates liver fibrosis through changes in TGF-β1 biliary secretion. Hepatology (Baltimore, Md.), 64(3), 865-879.

Publication 2016

Cells Hepatic stellate cells Human Mouse Neomycin Plasmid Short hairpin rna Vector

Corresponding Organization : Central Texas Veterans Health Care System

Other organizations : Baylor Medical Center at Garland, University of Milano-Bicocca, Humanitas University, Sapienza University of Rome, Marche Polytechnic University

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Variable analysis

independent variables

Stable transfection with vector or Sct in IMCL cells

dependent variables

Unknown, as the specific measured outcomes are not explicitly mentioned

control variables

IMCL control cells (stable transfected with the empty vector)
IMCL cells lacking Sct (established using shRNA)

controls

Positive control: IMCL cells stable-transfected with vector
Negative control: IMCL cells lacking Sct (established using shRNA)

Annotations

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