Protein Expression Analysis Protocol

Cells were homogenized in RIPA buffer as previously described (30 (link)). Antibodies for analyses included: RON (1:200, sc-322, Santa Cruz Biotechnology), Arginase-1 (1:1000, 610708, BD Transduction Labs), p-STAT3 (Y705) (1:800, 9145S, Cell Signaling Technology), STAT3 (1:500, 9139S, Cell Signaling Technology), and C4-actin (ACTIN) (1:40,000, Cincinnati Children’s Hospital Medical Center; 1:5000, sc-47778, Santa Cruz Biotechnology).

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Sullivan C., Brown N.E., Vasiliauskas J., Pathrose P., Starnes S.L, & Waltz S.E. (2020). Prostate Epithelial RON Signaling Promotes M2 Macrophage Activation to Drive Prostate Tumor Growth and Progression. Molecular cancer research : MCR, 18(8), 1244-1254.

Publication 2020

Arginase-1 p-STAT3 (Y705) STAT3 sc-47778

Actin Antibodies Arginase 1 Buffer Cells Ripa Stat3

Corresponding Organization : Veterans Health Administration

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

RON protein expression
Arginase-1 protein expression
Phosphorylation of STAT3 (Y705)
Total STAT3 protein expression
Actin (ACTIN) protein expression

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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