The change of myocardial structure and cytoplasmic distribution was evaluated by Haematoxylin-Eosin (HE) staining as described by Yu et al. [31 (link)]. Two normal rats (Con) and two diabetic rats (DM) were overnight fasted and sacrificed by rapid decapitation. All of the heart tissues were rapidly dissected, fixed overnight with 10% buffered neutral formalin, and embedded in paraffin after ethanol washing. The paraffin-embedded heart tissue was sliced into 5 μm sections, deparaffinised in xylene and ethanol series, and then washed in purified water. Finally, paraffin sections were stained according to standard protocols from the HE stain kit (Beyotime Institute of Biotechnology, Shanghai, China). The Eclipse 80i Fluorescence microscope (Nikon, Tokyo, Japan) was applied to capture the stained cardiac sections at 400× magnification. Then, we randomly selected 6 different sections to calculate percentages of normal myocardial cells in the total section area.
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