Ixofin3D was cloned in-frame into the pMT-Bip-V5-His tag vector (Invitrogen, CA) using ixofin3D_DESF and ixofin3D_DESR primers listed in Table S1 and recombinant protein expressed and purified using the Drosophila Expression System (Invitrogen, CA) as described earlier [22] (link). Recombinant protein purity was assessed by SDS-PAGE, and quantified using the BCA protein estimation kit (Thermoscientific, IL).
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