Immortalized B-Cell Line Generation

Whole blood was collected from six healthy Caucasian donors by Research Blood Components LLC (Brighton, MA) with IRB consent between 2009 and 2010. B-Cell isolation and LCL generation were performed at the University of Chicago as described previously [14 (link)]. Between February 2011 and October 2012, each line was thawed, cultured, and re-frozen every three months, for a total of six freeze-thaw cycles prior to use in our study [16 (link)]. LCLs were cultured in RPMI with 20% FBS and frozen in Recovery Cell Culture Freezing Media (Life Technologies).

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Thomas S.M., Kagan C., Pavlovic B.J., Burnett J., Patterson K., Pritchard J.K, & Gilad Y. (2015). Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature. PLoS Genetics, 11(5), e1005216.

Publication 2015

Recovery Cell Culture Freezing Media

B cell Blood Blood components Caucasian Cell Cell culture Cell isolation Culture media Donors Isolation Lcls

Corresponding Organization :

Other organizations : University of Chicago, Howard Hughes Medical Institute

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Variable analysis

independent variables

Number of freeze-thaw cycles (6 cycles)

dependent variables

Not explicitly mentioned

control variables

Caucasian ethnicity of donors
Time period of sample collection (2009-2010)
LCL culture conditions (RPMI with 20% FBS)
Cryopreservation media (Recovery Cell Culture Freezing Media)

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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