Antibacterial Efficacy of Hybrid Materials

Antibacterial properties of synthesized hybrid materials were evaluated against the Gram-negative Escherichia coli (ATCC 25922) and Gram-positive Enterococcus faecalis (ATCC 9212) bacteria. To this purpose, the bacterial culture was diluted in distilled water to produce a bacterial cell suspension of 10 × 10⁵ colony-forming unit (CFU)/mL. E. coli was inoculated in TBX Medium (Tryptone Bile X-Gluc) (Liofilchem, Roseto degli Abruzzi (TE), Italy), while E. faecalis was inoculated in Slanetz Bartley Agar Base (Liofilchem, Roseto degli Abruzzi (TE), Italy) in the presence of 100 mg of the hybrid powders. Afterward, the bacteria were incubated with the materials for 24 h at 44 °C and 48 h at 36 °C, respectively. The microbial growth was evaluated by observing the diameter of the inhibition halo (ID). The obtained values were expressed as mean ± standard deviation (SD) values of the measurements carried out in triplicate [24 (link)].

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Catauro M., Barrino F., Dal Poggetto G., Crescente G., Piccolella S, & Pacifico S. (2020). New SiO2/Caffeic Acid Hybrid Materials: Synthesis, Spectroscopic Characterization, and Bioactivity. Materials, 13(2), 394.

Publication 2020

TBX Medium (Tryptone Bile X-Gluc) Slanetz Bartley Agar Base

Agar Antibacterial Bacteria Bile Cell E coli Enterococcus faecalis Hybrid Inhibition Powders

Corresponding Organization : University of Campania "Luigi Vanvitelli"

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Variable analysis

independent variables

Hybrid powders (100 mg)

dependent variables

Diameter of the inhibition halo (ID)

control variables

Bacterial cell suspension concentration (10 × 10^5 CFU/mL)
Incubation time (24 h for E. coli, 48 h for E. faecalis)
Incubation temperature (44 °C for E. coli, 36 °C for E. faecalis)
Growth media (TBX Medium for E. coli, Slanetz Bartley Agar Base for E. faecalis)

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