Evaluating RAA Assay Specificity

Reference was made to published studies; four common and important DNA viruses were used to evaluate the specificity of the RAA assay [10 (link),50 (link)]. The five viruses are goat pox virus, sheep pox virus, African swine fever virus, bovine papular stomatitis virus and pseudorabies virus. African swine fever virus is stored in Biosafety Laboratory Level 3, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences. Viral RNA of the four viruses was extracted as a template to evaluate the specificity of the RAA assay.

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Cui H., Guan J., Lu H., Liu J., Tu F., Zhang C., Su K., Guo Z, & Zhao K. (2023). Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay. Life, 13(2), 494.

Publication 2023

African swine fever virus Assay Bovine Chinese Dna viruses Goat pox virus Pseudorabies virus Sheep pox virus Stomatitis Viral rna Virus

Corresponding Organization : Jilin University

Other organizations : Hebei Agricultural University

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Variable analysis

independent variables

Four common and important DNA viruses: goat pox virus, sheep pox virus, African swine fever virus, bovine papular stomatitis virus and pseudorabies virus

dependent variables

Specificity of the RAA assay

control variables

Viral RNA of the four viruses extracted as a template to evaluate the specificity of the RAA assay

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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