Transgenic Mice for Cardiac Studies

The BAC transgene Tbx5^CreERT2 was constructed from the BAC clone RP23-267B15^{70 (link)} by replacement of exon 2 of Tbx5 with a CreERT2 cassette at the first methionine of the open reading frame in EL250 cells^{23 (link),71 (link)}. The BAC-Tbx5^CreERT2 transgenes were crossed with Rosa26R^eYFP/eYFP transgenic mice (B6.129×1-Gt(ROSA)26Sortm1(EYFP)Cos/J) from Jackson laboratories stock# 006148, in order to produce Tbx5^CreERT2/Rosa26R^eYFP/eYFP mice employed in this study. The Tbx5^CreERT2/+/Rosa26R^eYFP/+/Rosa26R^iDTR/+ was created using the tamoxifen-induced Rosa26R^iDTR/iDTR transgene^{72 (link)} (Jackson laboratories stock# 007900) provided by the Klinakis lab (BRFAA).
All animal work has been approved by the BRFAA ethics committee and the Attica Veterinary Department (Animal Licence; 60876/23-1-20). All animals used were 2–3 months of age upon the time of ischemia/reperfusion (I/R) or isoproterenol administration experiments following relevant inclusion/exclusion guideline criteria^{73 (link)}.

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Siatra P., Vatsellas G., Chatzianastasiou A., Balafas E., Manolakou T., Papapetropoulos A., Agapaki A., Mouchtouri E.T., Ruchaya P.J., Korovesi A.G., Mavroidis M., Thanos D., Beis D, & Kokkinopoulos I. (2023). Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart. NPJ Regenerative Medicine, 8, 13.

Publication 2023

B6.129×1-Gt(ROSA)26Sortm1(EYFP)Cos/J

Animals Clone Ethics committee Exon Ischemia Isoproterenol Methionine Mice Reperfusion Rosa Tamoxifen Transgenes Transgenic mice

Corresponding Organization :

Other organizations : Academy of Athens, Biomedical Research Foundation of the Academy of Athens, National and Kapodistrian University of Athens, University of East London

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Variable analysis

independent variables

Tamoxifen-induced activation of the Rosa26R-iDTR/iDTR transgene

dependent variables

Unknown, not explicitly mentioned in the protocol

control variables

Age of animals (2-3 months) at the time of ischemia/reperfusion (I/R) or isoproterenol administration experiments
Inclusion/exclusion guideline criteria (as described in reference 73)

positive controls

BAC-Tbx5CreERT2 transgenic mice crossed with Rosa26R-eYFP/eYFP transgenic mice to produce Tbx5CreERT2/Rosa26R-eYFP/eYFP mice

negative controls

None explicitly mentioned

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