Antibiotic Susceptibility Testing Protocol

The present study provided the disks and powdered antibiotics from the MAST (Mast Diagnostics, United Kingdom) and Sigma-Aldrich (Taufkirchen, Germany). The following items were acquired from Merck (Merck, United States): Blood Agar, Mannitol Salt Agar (MSA), MacConkey agar, Cetrimide agar, Mueller Hinton Agar (MHA), DNA Agar, Mueller Hinton Broth (MHB), Trypticase Soy Broth (TSB), NaCl, glucose, and MgCl2. Fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal-Calf Serum (FCS), 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2 H-tetrazoliumbromide (MTT), Triton X-100, dimethyl sulfoxide (DMSO), agarose, ethanol, methanol, and crystal violet were provided from Sigma-Aldrich (Saint Louis, MO, United States). 96-well microplates, including flat-and round-bottom, were supplied by Jet Biofil (Guangzhou, China) and NEST Biotechnology (Wuxi, China), respectively.

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Mirzaei R., Esmaeili Gouvarchin Ghaleh H, & Ranjbar R. (2023). Antibiofilm effect of melittin alone and in combination with conventional antibiotics toward strong biofilm of MDR-MRSA and -Pseudomonas aeruginosa. Frontiers in Microbiology, 14, 1030401.

Publication 2023

Blood Agar Mannitol Salt Agar MacConkey agar Cetrimide agar Mueller Hinton Agar DNA Agar Mueller Hinton Broth Trypticase Soy Broth Triton X-100 dimethyl sulfoxide (DMSO), agarose ethanol methanol crystal violet

Agar Agarose Antibiotics Blood Cetrimide Crystal violet Diagnostics Dimethyl sulfoxide Diphenyl Eagle Ethanol Fetal calf serum Glucose Mannitol Methanol Mgcl2 Nacl Salt Triton x 100 Trypticase soy broth

Corresponding Organization : Baqiyatallah University of Medical Sciences

Other organizations : Hamedan University of Medical Sciences

Top 5 similar protocols

Variable analysis

independent variables

Powdered antibiotics from MAST (Mast Diagnostics, United Kingdom) and Sigma-Aldrich (Taufkirchen, Germany)

dependent variables

Measured outcomes

control variables

Blood Agar
Mannitol Salt Agar (MSA)
MacConkey agar
Cetrimide agar
Mueller Hinton Agar (MHA)
DNA Agar
Mueller Hinton Broth (MHB)
Trypticase Soy Broth (TSB)
Glucose
MgCl2
Fetal bovine serum (FBS)
Dulbecco's Modified Eagle's Medium (DMEM)
Fetal-Calf Serum (FCS)
3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2 H-tetrazoliumbromide (MTT)
Triton X-100
Dimethyl sulfoxide (DMSO)
Agarose
Ethanol
Methanol
Crystal violet
96-well microplates (flat-and round-bottom) from Jet Biofil (Guangzhou, China) and NEST Biotechnology (Wuxi, China)

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