MTHFR C677T Polymorphism in MI and DM

The current study was a case–control study that was conducted to determine the relationship between the frequencies of MTHFR C677T (rs1801133) gene mutation in MI patients in the presence of DM as a risk factor by analyzing the polymorphism using Tetra-ARMS PCR. The sampling was performed in the Allied Hospital, Faisalabad and the Faisalabad institute of Cardiology, Faisalabad, Pakistan, according to the guidelines approved by the ethical committee of Government College University, Faisalabad, Pakistan. We recruited a total of 300 study participants who were divided into three groups: Group I included the control patients (n = 100), group II included MI patients (n = 100), and group III (n = 100) included MI with diabetic patients (MI-DM). The study participants were briefly informed of the purpose of the research and written consent was obtained from all the participants before collecting blood. The blood specimen was collected from the target population and underwent various biochemical analyses and Tetra-ARMS PCR for genotyping to observe the genetic polymorphism.