Endothelin Receptor B Signaling Pathway

Endothelin 3 was purchased from Sigma Aldrich and reconstituted to 1 mg/ml in water. siRNA specific for EDNRB (sc-39962), along with control siRNA (sc-37007) were purchased from Santa Cruz (Santa Cruz Biotechnology, Santa Cruz, CA). EDNRB antibodies for Western blotting (ab117529) and flow cytometry (ab129102) and GAPDH antibody for Western blotting (ab9485) 31 (link) were purchased from Abcam (Cambridge, United Kingdom). pERK (#4370) and ERK (#4695) 32 (link) antibodies, along with pan AKT (4691), phospho AKT T308 (13038) and phospho-AKT1 S473 and phospho-AKT2 S474 (8599) were all purchased from Cell Signaling (Cell Signaling Technology, Beverly, MA). Secondary antibodies, including goat anti-mouse IgG-HRP, goat anti-rabbit IgG-HRP, goat anti-rabbit IgG-FITC were all purchased from Cell Signaling. Plasmid expression vectors encoding EDNRB-442 (RG 232943), EDNRB-536 (RC 216462), and EDNRB-436 (RC 225723) and accompanying empty vector controls (pCMV6-Entry vector) were purchased from Origene (Rockville, MD).

Free full text: Click here

Halaka M., Hired Z.A., Rutledge G.E., Hedgepath C.M., Anderson M.P., St. John H., Do J.M., Majmudar P.R., Walker C., Alawawdeh A., Stephen H.M., Reagor C.C., Adereti J., Jamison K., Iglesias K.P., Kirmani K.Z, & Conway R.E. (2020). Differences in Endothelin B Receptor Isoforms Expression and Function in Breast Cancer Cells. Journal of Cancer, 11(9), 2688-2701.

Publication 2020

Endothelin 3 control siRNA (sc-37007) ab117529 ab9485 goat anti-mouse IgG-HRP goat anti-rabbit IgG-HRP pCMV6-Entry vector)

Akt1 Akt2 Anti igg Antibodies Antibody Ednrb Endothelin 3 Fitc Flow cytometry Gapdh Goat Mouse Plasmid Rabbit Sirna Vector

Corresponding Organization :

Other organizations : Lipscomb University

Top 5 similar protocols

Variable analysis

independent variables

Endothelin 3 concentration (1 mg/ml in water)
SiRNA specific for EDNRB (sc-39962)
Control siRNA (sc-37007)
EDNRB expression vectors (EDNRB-442, EDNRB-536, EDNRB-436)
Empty vector controls (pCMV6-Entry vector)

dependent variables

EDNRB protein expression (measured by Western blotting and flow cytometry)
Activation of signaling pathways (pERK, ERK, pan AKT, phospho AKT T308, phospho-AKT1 S473 and phospho-AKT2 S474)

control variables

GAPDH antibody for Western blotting (ab9485)
Secondary antibodies (goat anti-mouse IgG-HRP, goat anti-rabbit IgG-HRP, goat anti-rabbit IgG-FITC)

controls

Positive control: Endothelin 3
Negative control: Control siRNA (sc-37007)
Negative control: Empty vector controls (pCMV6-Entry vector)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!