Positive ion mode (POS) : mobile phase A : 10 mM ammonium acetate, acetonitrile : water 95 : 5, .1% formic acid ; mobile phase B : 10 mM ammonium acetate, acetonitrile : water 50 : 50, 0.1% formic acid ; negative ion mode (NEG) : mobile phase A : 10 mM ammonium acetate, acetonitrile : water 95 : 5 ; adjust the pH to 8.0 with ammonia. Mobile phase B : 10 mM ammonium acetate, acetonitrile : water 50 : 50 ; adjust the pH to 8.0 with ammonia. The samples were separated by Nexera UHPLC LC-30A ultra-high performance liquid phase system with micro-liter flow rate. The chromatographic column was first balanced with 98% mobile phase A, and then the sample was transported by an automatic sampler to the HILIC column (Waters, ACQUITY UPLC BEH Amide 1.7 μm, 2.1 × 100 mm) at a flow rate of 0.3 mL/min.
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HILIC-MS Profiling of Metabolites
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Corresponding Organization :
Other organizations : Institute of Special Animal and Plant Sciences, The Affiliated Hospital to Changchun University of Chinese Medicine
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Variable analysis
independent variables
- Mobile phase A: 10 mM ammonium acetate, acetonitrile : water 95 : 5, .1% formic acid
- Mobile phase B: 10 mM ammonium acetate, acetonitrile : water 50 : 50, 0.1% formic acid
- Mobile phase A: 10 mM ammonium acetate, acetonitrile : water 95 : 5; adjust the pH to 8.0 with ammonia
- Mobile phase B: 10 mM ammonium acetate, acetonitrile : water 50 : 50; adjust the pH to 8.0 with ammonia
- Flow rate: 0.3 mL/min
- Chromatographic separation of the samples
- Nexera UHPLC LC-30A ultra-high performance liquid phase system
- HILIC column (Waters, ACQUITY UPLC BEH Amide 1.7 μm, 2.1 × 100 mm)
- Positive ion mode (POS)
- Negative ion mode (NEG)
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