Apoptosis Detection in Xenografted Tumors

GBC cells were cultured, and 1 × 10⁶ cells were collected. Annexin V / propidium iodide (PI) staining procedures were performed according to the following reference [34 (link)]. Apoptosis was detected with flow cytometer (FACS420, BD Biosciences, San Jose, CA, USA). Xenografted tumors in BALB/C nude mice were paraffin-embedded, sectioned and subjected to TUNEL assay (Maxim Biotech Inc., Fuzhou, China) to detect cell apoptosis [33 (link)]. The proportions of positive cells in each slice were counted within five high-power fields under microscope.

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Qiu Y., Li X., Yi B., Zheng J., Peng Z., Zhang Z., Wu M., Shen F, & Su C. (2015). Protein phosphatase PHLPP induces cell apoptosis and exerts anticancer activity by inhibiting Survivin phosphorylation and nuclear export in gallbladder cancer. Oncotarget, 6(22), 19148-19162.

Publication 2015

FACS420

Annexin v Apoptosis Assay Balb c mice Microscope Nude Paraffin Propidium iodide Tumors Tunel

Corresponding Organization : Xuzhou Medical College

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Variable analysis

independent variables

Cell culture of GBC cells

dependent variables

Apoptosis detected by flow cytometer (FACS420)
Apoptosis detected by TUNEL assay in xenografted tumors

control variables

Cell density of 1 × 10^6 GBC cells collected
Annexin V / propidium iodide (PI) staining procedures performed according to reference [34]
Xenografted tumors in BALB/C nude mice paraffin-embedded, sectioned and subjected to TUNEL assay according to reference [33]
Proportions of positive cells counted within five high-power fields under microscope

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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