Transcriptional Profiling of Epigenetic Regulators

RNeasy Mini Kit (Qiagen, Ontario, Toronto, Canada) was used for RNA extraction as per the manufacturer’s protocol. Preparation of cDNA and qRT-PCR were carried out as described previously [40 (link)-42 (link)]. Transcript levels of Mecp2 (total), Mecp2e1 [NCBI: NM_001081979.1], Mecp2e2 [NCBI: NM_010788.3], Dnmt genes (Dnmt1, Dnmt3a and Dnmt3b), neuronal genes (Tubulin III (Tub III), NeuN), astrocytic genes (Gfap, S100b), and oligodendrocyte-specific genes (Cnpase, Mbp) were examined by using gene-specific primers (Table 2), as described previously [37 (link),43 (link)]. The relative expression and fold changes were calculated as described previously [37 (link)]. Two-way analysis of variance (ANOVA) and the Student t-test were used to calculate significant differences between untreated control and decitabine-treated samples.

Free full text: Click here

Liyanage V.R., Zachariah R.M, & Rastegar M. (2013). Decitabine alters the expression of Mecp2 isoforms via dynamic DNA methylation at the Mecp2 regulatory elements in neural stem cells. Molecular Autism, 4, 46.

Publication 2013

Astrocytic Cdna Cnpase Decitabine Dnmt Dnmt1 Dnmt3b Gene Gfap Mecp2 Neuronal Oligodendrocyte Primers Student Tubulin

Corresponding Organization :

Other organizations : University of Manitoba

Top 5 similar protocols

Protocol cited in 5 other protocols

Variable analysis

independent variables

Treatment with decitabine

dependent variables

Transcript levels of Mecp2 (total), Mecp2e1, Mecp2e2, Dnmt genes (Dnmt1, Dnmt3a and Dnmt3b), neuronal genes (Tubulin III (Tub III), NeuN), astrocytic genes (Gfap, S100b), and oligodendrocyte-specific genes (Cnpase, Mbp)

control variables

Untreated control samples

controls

Untreated control samples

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!