Headspace SPME-GC/MS Analysis of Volatile Terpenes

Headspace SPME-GC/MS was employed to examine the volatile terpenes following our earlier studies [68 (link),69 (link),70 (link)]. Briefly, the 100 mm DVB/CAR/PDMS fused silica fibers (Sigma-Aldrich, St. Louis, MI, USA) were used to absorb the volatiles at 22 °C for 2 h. The volatiles captured by the fibers were subsequently thermally desorbed and analyzed by an Agilent 5975-6890N GC-MS apparatus (Agilent Technologies, Santa Clara, CA, USA) equipped with a HP-1MS fused-silica capillary column. Volatile terpenes were identified by comparing the mass spectra and retention times with those of standard samples or compounds deposited in the NIST 2008 mass spectra library. The relative content of volatile terpenes was quantitatively analyzed according to the standard curve of (E)-β-ocimene.