Chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated. N-succinimidyl ester conjugated (NHS)-Alexa647 and Cell trace violet (CTV) were from ThermoFisher Scientific (Waltham, MA, USA). α-Mannan from Malassezia furfur was purchased from InvivoGen (San Diego, CA, USA). Scleroglucan (β-glucan) was obtained from Elicityl (France). Lipopolysaccharide (LPS) from Klebsiella pneumoniae O1 was obtained from Dr. Chris Whitfield (University of Guelph, Canada). Alexa647-labeled anti-mouse Dectin-1 Ab (clone 2A11, rat IgG2b) was purchased from Bio-Rad (Hercules, CA, USA). Alexa647-labeled anti-Dectin-2 Ab (clone 2B4, rat IgG2a) was generated as described previously (11 (link)). Biotinylated anti-mouse Dectin-2 Ab (clone 2B4) was also generated using NHS-LC-biotin (ThermoFisher Scientific). Alexa647-labeled isotype-control Abs, mouse Fc receptor blocking Ab (clone 93, BioLegend), and R-phycoerythrin (PE)-labeled streptavidin were purchased from BioLegend (San Diego, CA, USA). ELISA kits for mouse TNFα and IL-1β were from BioLegend and R&D systems (Minneapolis, MN, USA), respectively. Anti-LC3 A/B (#4108S), β-actin (#4970S), and anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (#7074P2) were from Cell Signaling Technologies (Danvers, MA, USA).
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