Culturing HEK293 and MV^D7 cells

HEK293 cells were obtained from Simon Rothenfußer (Klinikum der Universitat München, München, Germany) as previously described (Menon et al., 2015 (link)). Cells were cultured in DMEM (Gibco cat. no. 11965092) with 10% FBS (Hyclone) and maintained at 5% CO₂ at 37°C. MV^D7 cells (Bear et al., 2000 (link)) were a gift from Frank Gertler (MIT, MA, USA). Cells were maintained in DMEM supplemented with GlutaMAX (Thermo Fisher Scientific, cat. no. 35050061), 15% FBS (Thermo Fisher cat. no. FB12999102), and 50 U/mL of interferon-γ (Sigma cat. no. IF005). Cells were cultured at 32°C with 5% CO₂. Cells lines are routinely tested for Mycoplasma by qPCR.