The HIV p24 concentration was measured as pictograms per milliliter
(pg/mL) using the commercially available Zeptometrix p24 ELISA kit. To determine
HIV concentration as molecules of HIV/mL, a ratio of p24 concentration (pg/mL)
to Nanoparticle Tracking Analysis (NTA; HIV/mL) was calculated for each HIV
preparation. NTA was performed using Nanosight NS300 (Malvern) as elaborated
earlier(16 (link)). HIVIG demonstrated
concentration-dependent Anti-p24 blocking; a range of HIVIG concentration
standards were used to correct all samples collected from mice injected with
HIV-HIVIG.
(pg/mL) using the commercially available Zeptometrix p24 ELISA kit. To determine
HIV concentration as molecules of HIV/mL, a ratio of p24 concentration (pg/mL)
to Nanoparticle Tracking Analysis (NTA; HIV/mL) was calculated for each HIV
preparation. NTA was performed using Nanosight NS300 (Malvern) as elaborated
earlier(16 (link)). HIVIG demonstrated
concentration-dependent Anti-p24 blocking; a range of HIVIG concentration
standards were used to correct all samples collected from mice injected with
HIV-HIVIG.
