Deep-Sea eDNA Metabarcoding Optimized with SuperFi II

We used a newly released PCR enzyme, Platinum SuperFi II DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA) (hereinafter referred to as "SuperFi II") in the first PCR for eDNA metabarcoding of deep-sea fish from pumped deep-sea water. The decision to use SuperFi II instead of KAPA HiFi HotStart DNA polymerase (Kapa Biosystems, Wilmington, MA, USA) (hereinafter referred to as "KAPA"), which was used in previous eDNA studies [11 (link),16 ,18 (link),19 ,31 (link),32] and introduced in the eDNA manual [33] , was based on its high sensitivity, inhibitor tolerance, and specificity in the amplification reaction, as described in the manufacturer's instruction.