Antibody Immunoblotting Protocol

Pex30 antibody (Joshi et al., 2016 (link)) was a kind gift from William Prinz (National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD). Dpm1 antibody (mouse monoclonal 5C5A7; dilution 1:10,000) was purchased from Invitrogen. HA antibody (rat monoclonal 3F10; dilution 1:2,000) was purchased from Roche. V5 antibody (rabbit monoclonal D3H8Q; dilution 1:5,000) was purchased from Cell Signaling. MYC antibody (mouse monoclonal 9E10; dilution 1:1,000) was purchased from Roche. LD dye BODIPY493/503 was purchased from Invitrogen and used at a final concentration of 1 µg/ml. MDH was purchased from Abgent and used at 0.1 mM.