MethylTarget™ assays were performed by Genesky BioTech (Shanghai, China) [12 (link), 23 (link)]. Briefly, we used an EZ DNA Methylation™-GOLD Kit (ZYMO RESEARCH) to transform all unmethylated cytosines to uracils. The samples with a bisulfite conversion rate < 98% were first filtered out. After the target CpG regions were amplified, separated, and purified by a gel extraction kit (Vazyme Biotech), a CpG island methylation assay was performed with an Illumina Hiseq/Miseq 2000 according to the manufacturer's protocol.
Li D., Yuan Y., Meng C., Lin Z., Zhao M., Shi L., Li M., Ye D., Cai Y., He X., Ye H., Zhou S., Zhou H, & Gao S. (2024). Low expression of miR-182 caused by DNA hypermethylation accelerates acute lymphocyte leukemia development by targeting PBX3 and BCL2: miR-182 promoter methylation is a predictive marker for hypomethylation agents + BCL2 inhibitor venetoclax. Clinical Epigenetics, 16, 48.
Other organizations :
First Affiliated Hospital of Wenzhou Medical University, Wenzhou Medical University, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
Bisulfite conversion rate (control for: ensure DNA is fully converted to uracil)
positive controls
Not explicitly mentioned
negative controls
Not explicitly mentioned
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