Previously described16 (link),32 (link) recombinant α5β1-Fc and trα5β1-Fc were generous gifts of Martin J. Humphries
(University of Manchester, UK),54 (link) αVβ3-Hexa-His was a kind gift of Junichi Takagi
(Osaka University, Japan).28 (link) α5β1-Poly-His was purchased from Sino Biological
(Wayne, PA). FN biotinylated at the N-terminus was a kind gift of
Andres J. Garcia (Georgia Institute of Technology, USA).55 (link) The anti-FN mAb (HFN7.1) was from Developmental
Studies Hybridoma Bank (Iowa City, IA). The antihuman Fc capturing
mAb (GG-7) was from Sigma-Aldrich (St. Louis, MO). LIBS-2 and BMC5
was purchased from EMD Millipore (Billerica, MA). Anti-Penta-His antibody
was purchased from Qiagen (Germany).
MAL-PEG3500-NHS and Biotin-PEG3500-NHS
were from JenKem (Plano, TX). Nystatin, streptavidin-maleimide, and
BSA were from Sigma-Aldrich. Borosilicate glass beads were from Distrilab
Particle Technology (RC Leusden, The Netherlands).
(University of Manchester, UK),54 (link) αVβ3-Hexa-His was a kind gift of Junichi Takagi
(Osaka University, Japan).28 (link) α5β1-Poly-His was purchased from Sino Biological
(Wayne, PA). FN biotinylated at the N-terminus was a kind gift of
Andres J. Garcia (Georgia Institute of Technology, USA).55 (link) The anti-FN mAb (HFN7.1) was from Developmental
Studies Hybridoma Bank (Iowa City, IA). The antihuman Fc capturing
mAb (GG-7) was from Sigma-Aldrich (St. Louis, MO). LIBS-2 and BMC5
was purchased from EMD Millipore (Billerica, MA). Anti-Penta-His antibody
was purchased from Qiagen (Germany).
MAL-PEG3500-NHS and Biotin-PEG3500-NHS
were from JenKem (Plano, TX). Nystatin, streptavidin-maleimide, and
BSA were from Sigma-Aldrich. Borosilicate glass beads were from Distrilab
Particle Technology (RC Leusden, The Netherlands).
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