Sensitivity and specificity of diagnostic tests were calculated according to the following formulas: SE = TP/(TP + FN)*100, SP = TN/(TN + FP)*100; PPV, NPV and accuracy was calculated as follows: PPV = TP/P, NPV = TN/N, Accuracy = TP/(TP + TN)*100. FN: False negative; FP: False positive; N: Negative; NPV: Negative predict value; P: Positive; PPV: Positive predict value; SE: Sensitivity; SP: Specificity; TN: True negative; TP: True positive.
All of presented dHMGS dye intensity traces show a representative outcome form an individual run from gastric biopsy specimen selected from total dataset subjected to analysis. Data were statistically analyzed by χ2 test using Stata statistical software package version 12.0 (StataCorp College Station, TX, USA). Additionally, Gwet’s AC1 values were calculated to measure the detection agreement of dHMGS assay with sequencing using AgreeStat version 2011.3 (Advanced Analytics, MD, USA) [8 (link)]. The benchmark scales for Gwet’s AC1 values was based on the Landis and Koch’s criteria [9 (link)]. The H. pylori distribution of different groups was analyzed by Mann–Whitney rank-sum test for two variables and Kruskal–Wallis H test for more than two variables. All of the above hypothesis tests were two-sided; and a two-tailed p-value of 0.05 or less was considered to indicate statistical significance.
All of presented dHMGS dye intensity traces show a representative outcome form an individual run from gastric biopsy specimen selected from total dataset subjected to analysis. Data were statistically analyzed by χ2 test using Stata statistical software package version 12.0 (StataCorp College Station, TX, USA). Additionally, Gwet’s AC1 values were calculated to measure the detection agreement of dHMGS assay with sequencing using AgreeStat version 2011.3 (Advanced Analytics, MD, USA) [8 (link)]. The benchmark scales for Gwet’s AC1 values was based on the Landis and Koch’s criteria [9 (link)]. The H. pylori distribution of different groups was analyzed by Mann–Whitney rank-sum test for two variables and Kruskal–Wallis H test for more than two variables. All of the above hypothesis tests were two-sided; and a two-tailed p-value of 0.05 or less was considered to indicate statistical significance.
