Tracking HA-iMSC-EVs Biodistribution

HA-iMSC-EVs were incubated with 1 μg/mL DiR or DiD buffer according to the protocol provided by Lipophilic Tracers (Invitrogen, Waltham, MA, USA) as described previously [34 (link)]. 500 μg of DiR-labeled HA-iMSC-EVs were resuspended in 0.05 mL of phosphate buffered saline (PBS) and intravenously or intramyocardially injected into rats. DiR-labeled HA-iMSC-EVs were detected at 6 and 24 h using an In Vivo Imaging System (IVIS, Perkin Elmer, Waltham, MA, USA). Human umbilical venous endothelial cells (HUVECs) or neonatal rat cardiac fibroblasts (NRCFs) were treated with DiD-labeled HA-iMSC-EVs. After 24 h, DiD-labeled HA-iMSC-EVs were observed under a Nikon Eclipse Ti2-U fluorescence microscope (Nikon, Tokyo, Japan).

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Jeong S.Y., Park B.W., Kim J., Lee S., You H., Lee J., Lee S., Park J.H., Kim J., Sim W., Ban K., Park J., Park H.J, & Kim S. (2024). Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct. Journal of Nanobiotechnology, 22, 149.

Publication 2024

Lipophilic Tracers In Vivo Imaging System (IVIS, Nikon Eclipse Ti2-U fluorescence microscope

Corresponding Organization :

Other organizations : Catholic University of Korea, City University of Hong Kong, Seoul National University

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Variable analysis

independent variables

Incubation of HA-iMSC-EVs with 1 μg/mL DiR or DiD buffer
Intravenous or intramyocardial injection of 500 μg of DiR-labeled HA-iMSC-EVs
Treatment of HUVECs or NRCFs with DiD-labeled HA-iMSC-EVs

dependent variables

Detection of DiR-labeled HA-iMSC-EVs at 6 and 24 h using IVIS
Observation of DiD-labeled HA-iMSC-EVs in HUVECs or NRCFs after 24 h using a fluorescence microscope

control variables

Phosphate buffered saline (PBS) used for resuspension of DiR-labeled HA-iMSC-EVs

controls

Positive control: DiR-labeled HA-iMSC-EVs
Negative control: Not explicitly mentioned

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