Quantifying Protein-Nucleosome Interactions
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Chinese Academy of Sciences
Other organizations : Van Andel Institute, University of Southern California
Variable analysis
- Concentration of biotinylated nucleosomes (100 nM)
- Concentration of His-tagged proteins (100 nM)
- Photon counts measured using an Envision-Alpha Reader
- Volume of the reaction (100 μL)
- Composition of the AlphaScreen buffer (50 mM MOPS, pH 7.4, 50 mM NaF, 50 mM CHAPS, and 0.1 mg/mL BSA)
- Concentration of streptavidin-coated donor beads (5 μg/mL)
- Concentration of nickel-chelated acceptor beads (5 μg/mL)
- Incubation time (1.5 h)
- Incubation temperature (room temperature)
- Incubation conditions (in the dark)
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!