The following technologies and materials were used in this research: UV spectra (Shimadzu, Kyoto, Japan); IR spectra (Bruker Vector 22 spectrophotometer, Bruker Optics GmbH, Ettlingen, Germany); Mass spectra (API QSTAR time-of-flight spectrometer, MDS Sciqaszex, Concord, Ontario, Canada). NMR spectra (Bruker AM-400, Bremerhaven, Germany); silica gel (200–300 and 300–400 mesh, Qingdao Marine Chemical Inc., China); Rp-18 gel (40–63 μm, Merck, Darmstadt, Germany); Sephadex LH-20 (20–150 μm, Amersham Biosciences, Uppsala, Sweden); YMC*GEL ODS-A-HG (50 μm, YMC Co. Ltd., Kyoto, Japan) (34 (link)).
The following reagents were used in this research: FBS (Gibco, Grand Island, NE, USA); DMEM, neutral red (Solarbio, Beijing, China); Nitric oxide kit (Nanjing Jiancheng Bioengineering Institute); IL-6 and TNF-α ELISA kit (Beijing 4A Biotech Co., Ltd., Beijing, China); Primer iNOS, TNF-α, IL-6 and COX-2 (Thermo Fisher Scientific, Shanghai, China); Reactive Oxygen Species Assay Kit (Beyotime Biotechnology, Shanghai, China); PrimeScriptTMRT reagent kit with gDNA Eraser kit and TB Green TM Ex TaqTM II (Tli RNadeH Plus, Accurate Biology, Hunan, China), Bulk kit (TaKaRa, Accurate Biology, Hunan, China); Antibody NF-κB p65, phospho-NF-κB p65, iNOS, COX-2, IκBα, and phospho-IκBα (Cell Signaling, Beverly, MA, USA); LPS (Sigma-Aldrich, St. Louis, MO, USA) (35 (link)).
The following reagents were used in this research: FBS (Gibco, Grand Island, NE, USA); DMEM, neutral red (Solarbio, Beijing, China); Nitric oxide kit (Nanjing Jiancheng Bioengineering Institute); IL-6 and TNF-α ELISA kit (Beijing 4A Biotech Co., Ltd., Beijing, China); Primer iNOS, TNF-α, IL-6 and COX-2 (Thermo Fisher Scientific, Shanghai, China); Reactive Oxygen Species Assay Kit (Beyotime Biotechnology, Shanghai, China); PrimeScriptTMRT reagent kit with gDNA Eraser kit and TB Green TM Ex TaqTM II (Tli RNadeH Plus, Accurate Biology, Hunan, China), Bulk kit (TaKaRa, Accurate Biology, Hunan, China); Antibody NF-κB p65, phospho-NF-κB p65, iNOS, COX-2, IκBα, and phospho-IκBα (Cell Signaling, Beverly, MA, USA); LPS (Sigma-Aldrich, St. Louis, MO, USA) (35 (link)).
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