Tick Salivary Gland Extraction and Quantification

Pathogen-free Ixodes scapularis ticks were maintained as described previously (15 (link)). The salivary glands from pathogen-free I. scapularis adult females were dissected and stored in phosphate-buffered saline (PBS). The salivary glands were homogenized and quantified using the micro-bicinchoninic acid protein assay kit (Thermo Scientific).

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Hermance M.E, & Thangamani S. (2015). Tick Saliva Enhances Powassan Virus Transmission to the Host, Influencing Its Dissemination and the Course of Disease. Journal of Virology, 89(15), 7852-7860.

Publication 2015

micro-bicinchoninic acid protein assay kit

Adult Assay Bicinchoninic acid Females Ixodes scapularis Pathogen Phosphate Protein Saline Salivary glands Ticks

Corresponding Organization :

Other organizations : The University of Texas Medical Branch at Galveston

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Protocol cited in 4 other protocols

Variable analysis

independent variables

Pathogen-free Ixodes scapularis ticks

dependent variables

Salivary gland protein concentration

control variables

Pathogen-free I. scapularis adult females
Phosphate-buffered saline (PBS)

controls

Pathogen-free I. scapularis ticks

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