Histological Analysis of Cardiac Tissue

Histological analysis was carried out as we previously described [40 (link)]. Briefly, hearts were excised, fixed in 10% formalin, embedded in paraffin and sectioned into 4-μm slices. The slides were stained with hematoxylin-eosin (H&E) and examined under a light microscope. The cross-sectional area of cardiomyocytes was determined by staining with FITC-conjugated wheat germ agglutinin (Sigma). Cardiac fibrosis was assessed by standard Masson trichrome staining (Sigma) following manufacturer’s instructions.

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Ye X., Hang Y., Lu Y., Li D., Shen F., Guan P., Dong J., Shi L, & Hu W. (2021). CircRNA circ-NNT mediates myocardial ischemia/reperfusion injury through activating pyroptosis by sponging miR-33a-5p and regulating USP46 expression. Cell Death Discovery, 7, 370.

Publication 2021

FITC-conjugated wheat germ agglutinin Masson trichrome staining

Cardiac Cardiomyocytes Embedded paraffin Eosin Fibrosis Fitc Formalin Microscope light Wheat germ agglutinin

Corresponding Organization :

Other organizations : Minhang District Central Hospital, Fudan University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cross-sectional area of cardiomyocytes
Cardiac fibrosis

control variables

Tissue processing (excision, fixation, embedding, sectioning)
Staining techniques (hematoxylin-eosin, FITC-conjugated wheat germ agglutinin, Masson trichrome)

controls

Positive control: None mentioned
Negative control: None mentioned

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