Protocol detail

Cultivation and Characterization of G. lucidum Strains

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G. lucidum is a species complex that shows tremendous intra-species diversity43 (link). The G. lucidum dikaryotic strain CGMCC5.0026, belonging to the G. lucidum Asian group, was obtained from the China General Microbiological Culture Collection Center (Beijing, China) and is one of the most widely used isolates for the production of G. lucidum medicinal material in China. The monokaryotic strain G.260125-1 used for whole-genome sequencing was derived from the strain CGMCC5.0026 by protoplasting. Vegetative mycelia were grown on potato dextrose medium in the dark at 28 °C. Liquid cultures were shaken at 50 r.p.m. The primordia and fruiting bodies of the strain CGMCC5.0026 used for transcriptomic analyses were cultivated on Quercus variabilis Blume logs at HuiTao Pharmaceutical Company (LuoTian, Hubei Province, China). All strains are available on request.

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Chen S., Xu J., Liu C., Zhu Y., Nelson D.R., Zhou S., Li C., Wang L., Guo X., Sun Y., Luo H., Li Y., Song J., Henrissat B., Levasseur A., Qian J., Li J., Luo X., Shi L., He L., Xiang L., Xu X., Niu Y., Li Q., Han M.V., Yan H., Zhang J., Chen H., Lv A., Wang Z., Liu M., Schwartz D.C, & Sun C. (2012). Genome sequence of the model medicinal mushroom Ganoderma lucidum. Nature Communications, 3, 913-.

Publication 2012

Asian Bodies Dextrose Mycelia Pharmaceutical Potato Quercus Strains Transcriptomic analyses

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Variable analysis

independent variables

Protoplasting of the dikaryotic strain CGMCC5.0026 to derive the monokaryotic strain G.260125-1 used for whole-genome sequencing

dependent variables

Whole-genome sequence of the monokaryotic strain G.260125-1

control variables

Vegetative mycelia were grown on potato dextrose medium in the dark at 28 °C
Liquid cultures were shaken at 50 r.p.m.
Primordia and fruiting bodies of the strain CGMCC5.0026 were cultivated on Quercus variabilis Blume logs

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