Immunostaining for Calbindin-Expressing Neurons

To reveal neurons containing the calcium-binding protein calbindin, we used the CB38a anti-calbindin rabbit polyclonal antibody from Swant (CB38a, Swant; RRID AB_10000340) at a dilution of 1:10000. The pattern of immunoreactivity throughout the gibbon brain was similar to that seen in other mammals (e.g. Bhagwandin et al., 2013 (link); Bunce, Zikopoulos, Feinberg, & Barbas, 2013 (link); Gritti et al., 2003 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Swiegers J., Bhagwandin A., Sherwood C.C., Bertelsen M.F., Maseko B.C., Hemingway J., Rockland K.S., Molnár Z, & Manger P.R. (2018). The distribution, number and certain neurochemical identities of infracortical white matter neurons in a lar gibbon (Hylobates lar) brain.. The Journal of comparative neurology, 527(10), 1633-1653.

Publication 2018

CB38a

Anti antibody Brain Calbindin Calcium binding protein Dilution Gibbon Mammals Neurons Rabbit Seen

Corresponding Organization : University of the Witwatersrand

Other organizations : George Washington University, Copenhagen Zoo, Boston University, University of Oxford

Top 5 similar protocols

Variable analysis

independent variables

CB38a anti-calbindin rabbit polyclonal antibody

dependent variables

Pattern of immunoreactivity throughout the gibbon brain

control variables

Kept constant to ensure valid results (not explicitly mentioned)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!