Assay for CD8+ T Cell Adhesion
Corresponding Organization :
Other organizations : University of Iowa, George Washington University, Hackensack University Medical Center, Center for Discovery, University of Minnesota, Minneapolis VA Health Care System, University of Minnesota Medical Center
Variable analysis
- Concentration of αCD3 (0–10 μg)
- Cellular adhesion
- Flat-bottomed 96-well plates (Thermo-Fisher)
- Incubation time (30 min)
- P14 CD8 T cells isolated by positive selection, based on Thy1.1
- Staining with αCD8a-APC-Cy7 (53–6.7; Biolegend)
- Washing with PBS before imaging
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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