Transfection of miRNA and siRNA in PDAC

Pre-miR miRNA precursors and siRNAs were purchased from Thermo Fisher Scientific, as follows: miR-124-3p (product ID: PM 10245), negative control miRNA (product ID: AM 17111), two ITGA3 siRNAs (product IDs: HSS105529 and HSS179967), two ITGB1 siRNAs (product IDs: HSS105559 and HSS105561), and negative control siRNA (product ID: D-001810-10). Lipofectamine RNAiMAX and Lipofectamine 2000 (Thermo Fisher Scientific) were used for transfection into PDAC cell lines. The transfection efficiencies of miRNA in cancer cells were calculated as described in previous studies [42 (link)].

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Idichi T., Seki N., Kurahara H., Fukuhisa H., Toda H., Shimonosono M., Yamada Y., Arai T., Kita Y., Kijima Y., Mataki Y., Maemura K, & Natsugoe S. (2018). Involvement of anti-tumor miR-124-3p and its targets in the pathogenesis of pancreatic ductal adenocarcinoma: direct regulation of ITGA3 and ITGB1 by miR-124-3p. Oncotarget, 9(48), 28849-28865.

Publication 2018

miR-124-3p Lipofectamine RNAiMAX Lipofectamine 2000

Cancer Cell lines Cells Lipofectamine Lipofectamine 2000 Mir 124 Mirna Pdac Pre mirna Sirna Transfection

Corresponding Organization : Chiba University

Other organizations : Kagoshima University

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Protocol cited in 7 other protocols

Variable analysis

independent variables

MiR-124-3p miRNA precursor
Negative control miRNA
ITGA3 siRNAs (two)
ITGB1 siRNAs (two)
Negative control siRNA

dependent variables

Transfection efficiencies of miRNA in cancer cells

control variables

Lipofectamine RNAiMAX and Lipofectamine 2000 for transfection

controls

Negative control miRNA
Negative control siRNA

Annotations

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