Expansion of Three MSC Types

Three types of MSCs were expanded as previously reported [16 (link), 17 (link)]. BMMSCs (Cat no. PT-2501, Lonza, USA) were maintained in DMEM containing 10% FBS and 1% antibiotic-penicillin/streptomycin solution. ADMSCs (Cat no. R7788-115, Invitrogen) were expanded using MesenPRO RS™ basal medium, growth supplements and 2% FBS. SFMSCs were plated in complete culture medium consisting of α -MEM (Invitrogen) with 10% FBS, 1% antibiotic-penicillin/streptomycin solution and 250 ng/ml amphotericin B (Invitrogen). All types of cells were incubated at 37 °C under a humidified atmosphere containing 5% CO₂.

Free full text: Click here

Kim R., Park S.I., Lee C.Y., Lee J., Kim P., Oh S., Lee H., Lee M.Y., Kim J., Chung Y.A., Hwang K.C., Maeng L.S, & Chang W. (2016). Alternative new mesenchymal stem cell source exerts tumor tropism through ALCAM and N-cadherin via regulation of microRNA-192 and -218. Molecular and Cellular Biochemistry, 427(1), 177-185.

Publication 2016

BMMSCs ADMSCs MesenPRO α -MEM amphotericin B

Amphotericin b Antibiotic Atmosphere Cells types Culture medium Penicillin Streptomycin Supplements

Corresponding Organization :

Other organizations : Pusan National University, Incheon St. Mary's Hospital, Catholic University of Korea, Yonsei University, Stanford University, Yale University, Kyungpook National University, Sookmyung Women's University, Catholic Kwandong University

Top 5 similar protocols

Variable analysis

independent variables

Three types of MSCs (BMMSCs, ADMSCs, SFMSCs)

dependent variables

Not explicitly mentioned

control variables

Culture media (DMEM with 10% FBS and 1% antibiotic-penicillin/streptomycin for BMMSCs, MesenPRO RS™ basal medium with growth supplements and 2% FBS for ADMSCs, α-MEM with 10% FBS, 1% antibiotic-penicillin/streptomycin, and 250 ng/ml amphotericin B for SFMSCs)
Incubation conditions (37 °C, 5% CO2, humidified atmosphere)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!