Leucostasis in diabetic mice was assessed after 4 weeks of diabetes using fluorophore-conjugated concanavalin-A, as described previously [16 (link)]. The retinas were mounted on slides and visualised using a Nikon TE EZ-C1 confocal system (Nikon). Two z stacks of the retina for each artery/vein with the capillaries (central and peripheral) were taken and the total number of leucocytes in the arteries, veins and capillaries were counted.
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