Individual flies were attached to a custom-milled sample plate using
double-sided tissue tape (7111, Louis Adhesive Tapes, Thailand). No matrix was
used. Mass spectra were generated using a QSTAR Elite (AB Sciex, Toronto, CA)
mass spectrometer equipped with a modified oMALDI2 ion source (AB Sciex) and a
N2 laser (λ = 337 nm) operated at a
repetition rate of 40 Hz.9 (link),20 (link) Two mbar of N2 gas
was used create the buffer gas environment for generation of ions. [M +
K]+ potassium-bearing compounds constitute the major ion species.
Spectra were internally calibrated to chitin signals at [M + K]+242.04, 648.20 and 851.28. Mass accuracy was ~20 ppm All spectra were
acquired in positive ion mode and processed using MS Analyst software (Analyst
QS 2.0, AB Sciex).
double-sided tissue tape (7111, Louis Adhesive Tapes, Thailand). No matrix was
used. Mass spectra were generated using a QSTAR Elite (AB Sciex, Toronto, CA)
mass spectrometer equipped with a modified oMALDI2 ion source (AB Sciex) and a
N2 laser (λ = 337 nm) operated at a
repetition rate of 40 Hz.9 (link),20 (link) Two mbar of N2 gas
was used create the buffer gas environment for generation of ions. [M +
K]+ potassium-bearing compounds constitute the major ion species.
Spectra were internally calibrated to chitin signals at [M + K]+242.04, 648.20 and 851.28. Mass accuracy was ~20 ppm All spectra were
acquired in positive ion mode and processed using MS Analyst software (Analyst
QS 2.0, AB Sciex).
