ChIP-exo was performed using the ChIP-exo 5.0 protocol (39 (link)) with minor adaptations. Protein A Mag Sepharose (GE Healthcare) beads were preblocked and bound with antibody before the ChIP. Ten microliters of beads was washed three times with 500 μl of 0.5% BSA in 1× PBS at 4°C. Five micrograms of antibody was added to the washed beads, and the beads were resuspended in 250 μl of the 0.5% BSA solution and allowed to incubate overnight on a rotating platform at 4°C. The next day, the beads were washed three times in 500 μl of 0.5% BSA solution. Sixty micrograms of chromatin was added to the beads, and the IP was performed in a total volume of 500 μl of IP dilution buffer [20 mM tris-HCl (pH 8.0), 2 mM EDTA, 150 mM NaCl, and 1% Triton X-100] with protease inhibitors (Roche) at 4°C overnight. A total of 120 μg of chromatin (two samples with 60 μg of chromatin) was used for each ChIP-exo experiment. In addition, NEBNext Multiplex Oligos for Illumina Index Primers and the Universal PCR Primer for Illumina were used instead of the ExA2_iNN and the ExA1-58 oligos. All other steps were identical to the original ChIP-exo 5.0 protocol.