Quantifying Plasma C3c Complement Binding
Corresponding Organization :
Other organizations : Mario Negri Institute for Pharmacological Research, Istituti di Ricovero e Cura a Carattere Scientifico
Variable analysis
- Plasma dilutions (10%, 5%, 2.5%, 1.25%, and 0.75% in BBS)
- Coating of plates (mannan- (10 µg/mL) or acBSA- (25 µg/mL))
- Complement C3c binding measured by absorbance at 405 nm
- Incubation time (15 min)
- Incubation temperature (Room Temperature)
- Anti-human-C3c antibody concentration (2.4 µg/mL)
- Anti-rabbit IgG antibody concentration (1 µg/mL)
- Pooled plasma of mice belonging to the same experimental group
- Not explicitly mentioned
Annotations
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