Intestinal Inflammatory Protein Analysis

Immunoblotting was performed as described previously [16 (link)]. Briefly, dissected intestinal were homogenized and lysed with lysis buffer. Protein extract was separated on SDS-PAGE and transferred to polyvinylidenedifluoride (PVDF) membrane and probed with the following antibodies: IL-6, IL-1β, TNF-α, PGE2, COX-2, and β-actin from Santa Cruz Biotechnology (Santa Cruz, CA). Band intensity was determined by densitometry analysis using the ImageJ analysis system.

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Park S.A., Lee G.H., Hoang T.H., Lee H.Y., Kang I.Y., Chung M.J., Jin J.S, & Chae H.J. (2021). Heat-inactivated Lactobacillus plantarum nF1 promotes intestinal health in Loperamide-induced constipation rats. PLoS ONE, 16(4), e0250354.

Publication 2021

IL-6 IL-1β TNF-α COX-2 β-actin

Actin Antibodies Buffer Cox 2 Densitometry Il 1β Intestinal Membrane Pge2 Polyvinylidenedifluoride Protein Sds page Tnf α

Corresponding Organization : Jeonbuk National University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of IL-6, IL-1β, TNF-α, PGE2, and COX-2

control variables

β-actin expression (used as a loading control)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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