Differentiation of CuFi-8 cells into Air-Liquid Interface Culture

CuFi-8 cells differentiate on collagen-coated, semipermeable membrane inserts (0·6 cm², Millicell-PCF; Millipore, Billerica, MA) into an air-liquid interface culture. After 3–4 weeks of culture in DMEM:F12 medium (50%:50%) containing 2% Ultroser G (Pall BioSepra, Cergy-Staint-Christophe, France) the polarity of the HAE was determined based on the transepithelial electrical resistance (TEER) using an epithelial Volt-Ohm Meter (Millipore). HBoV1 infection was performed at minimal values of 600–800 Ω per insert [27 (link)].

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Khalfaoui S., Eichhorn V., Karagiannidis C., Bayh I., Brockmann M., Pieper M., Windisch W., Schildgen O, & Schildgen V. (2016). Lung Infection by Human Bocavirus Induces the Release of Profibrotic Mediator Cytokines In Vivo and In Vitro. PLoS ONE, 11(1), e0147010.

Publication 2016

Millicell-PCF Volt-Ohm Meter

Cells Collagen Electrical resistance Infection Membrane Ultroser g

Corresponding Organization : Witten/Herdecke University

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Variable analysis

independent variables

Cell differentiation on collagen-coated, semipermeable membrane inserts
Air-liquid interface culture
Duration of culture (3-4 weeks)
DMEM:F12 medium (50%:50%) containing 2% Ultroser G
HBoV1 infection

dependent variables

Transepithelial electrical resistance (TEER)

control variables

Semipermeable membrane insert size (0.6 cm^2)
Minimal TEER values (600-800 Ω per insert)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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