Immunohistochemical Analysis of ANGPTL8, α-SMA, and MAC-2

Immunohistochemical staining was performed as previously described [22 (link)] using primary antibodies against ANGPTL8 (1:200 dilution; Abcam, Cambridge, UK), α-smooth muscle actin (α-SMA) (1:200 dilution; ZM0003, ZSGB-BIO, Beijing, China), or galectin 2 (MAC-2) (1:200 dilution; Abcam, Cambridge, UK), followed by staining with secondary antibodies. Images were obtained with a Ni-UNikon Upright Microscope equipped with a DS-Ri2 color CCD (Nikon, Tokyo, Japan).

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Yang Y., Jiao X., Li L., Hu C., Zhang X., Pan L., Yu H., Li J., Chen D., Du J, & Qin Y. (2020). Increased Circulating Angiopoietin-Like Protein 8 Levels Are Associated with Thoracic Aortic Dissection and Higher Inflammatory Conditions. Cardiovascular Drugs and Therapy, 34(1), 65-77.

Publication 2020

ANGPTL8 MAC-2 DS-Ri2 color CCD

Actin Antibodies Dilution Galectin 2 Microscope Smooth muscle

Corresponding Organization :

Other organizations : Beijing Anzhen Hospital, Capital Medical University

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Variable analysis

independent variables

Primary antibodies against ANGPTL8 (1:200 dilution)
Primary antibodies against α-smooth muscle actin (α-SMA) (1:200 dilution)
Primary antibodies against galectin 2 (MAC-2) (1:200 dilution)

dependent variables

Immunohistochemical staining patterns

control variables

Secondary antibodies used for staining
Microscope equipment used for imaging (Ni-UNikon Upright Microscope equipped with a DS-Ri2 color CCD)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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