Paraffin Sectioning and Staining for Plant Samples

Paraffin sections of 10-DAP kernels were prepared according to a previously described protocol [73 (link)]. The samples were treated with xylene, embedded in paraffin wax via infiltration, and cut into 10 μm-thick sections with a Thermo Scientific HM 325 rotary microtome. The sections were stained with toluidine blue (Sinopharm Chemical Reagent Co., Ltd) and examined under a ZEISS Stemi 508 Stereo Microscope (Germany) and a ZEISS Axio Scope A1 Microscope (Germany).

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Tian R., Jiang J., Bo S., Zhang H., Zhang X., Hearne S.J., Tang J., Ding D, & Fu Z. (2023). Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development. BMC Plant Biology, 23, 191.

Publication 2023

toluidine blue Stemi 508 Stereo Microscope Axio Scope A1 Microscope

Microscope Microtome Paraffin wax Stemi Toluidine blue Xylene

Corresponding Organization :

Other organizations : Henan Agricultural University, Centro Internacional de Mejoramiento de Maíz Y Trigo

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Variable analysis

independent variables

Paraffin sections of 10-DAP kernels

dependent variables

Morphological examination of the sections under a ZEISS Stemi 508 Stereo Microscope and a ZEISS Axio Scope A1 Microscope

control variables

Paraffin sections were prepared according to a previously described protocol
Sections were cut into 10 μm-thick sections with a Thermo Scientific HM 325 rotary microtome
Sections were stained with toluidine blue (Sinopharm Chemical Reagent Co., Ltd)

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