Antipsychotics Effects on Poly(I:C) Activation

Poly (I:C) was purchased from InvivoGen (San Diego, CA, USA). Clozapine, risperidone, haloperidol, and CRID3 (CP-456, 773) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The antipsychotics risperidone (20 μM), Clozapine (10 μM), and haloperidol (20 μM) were dissolved in dimethyl sulfoxide (DMSO) with a final concentration of 0.05% in the culture medium [18 (link),19 (link)]. DMSO at the highest concentration (0.05%) that was used for the experimental conditions was not toxic to the cells.

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V. Giridharan V., Scaini G., Colpo G.D., Doifode T., F. Pinjari O., Teixeira A.L., Petronilho F., Macêdo D., Quevedo J, & Barichello T. (2020). Clozapine Prevents Poly (I:C) Induced Inflammation by Modulating NLRP3 Pathway in Microglial Cells. Cells, 9(3), 577.

Publication 2020

Poly (I:C) Clozapine risperidone haloperidol

Antipsychotics Cells Clozapine Crid3 Culture medium Dimethyl sulfoxide Haloperidol Poly i c Risperidone

Corresponding Organization : Universidade do Sul de Santa Catarina

Other organizations : Universidade Federal do Ceará, National Council for Scientific and Technological Development, The University of Texas MD Anderson Cancer Center

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Variable analysis

independent variables

Poly (I:C)
Clozapine
Risperidone
Haloperidol
CRID3 (CP-456, 773)

dependent variables

Not explicitly mentioned

control variables

DMSO concentration (0.05%)
Cell toxicity

controls

DMSO at the highest concentration (0.05%) that was used for the experimental conditions was not toxic to the cells.

Annotations

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