Total RNA was extracted from rat hearts with Trizol reagent (Invitrogen, Life Technologies, Carlsbad, CA) and reverse-transcribed into cDNA with a specific RT primer (TaKaRa, Dalian, China). Real-time PCR assays were performed using SYBR Green (Bimake, Shanghai, China) with specific primers on a 7900HT FAST real-time PCR system (Life Technologies, Carlsbad, CA). Data analysis was performed by the 2−ΔΔCt method, as described previously (28 (link)). The primers are listed in Supplementary Table 1 .
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