Cell Treatment with Chemical Modulators

PMA (Sigma-Aldrich, P8139) was added to media at 25 ng ml⁻¹ at 37 °C with 5% CO₂ for denoted time. JQ1 (Tocris, 4499) was added to media at 0.5 μM at 37 °C with 5% CO₂ for 4 h unless denoted otherwise. Triptolide (Tocris, 3253; Selleckchem, S3604) was added to media at 1 μM at 37 °C with 5% CO₂ for 4 h unless denoted otherwise. Flavopiridol (Selleckchem, S1230) was added to media at 1 μM at 37 °C with 5% CO₂ for 1 h unless denoted otherwise. Dox (MP Biomedicals, 198955) was added to media at 1 μg ml⁻¹ at 37 °C with 5% CO₂ for 24 h unless denoted otherwise. The splicing inhibitor, Plad B (ref. ^{54 (link)}; Tocris, 6070), was added to media at 5 μM at 37 °C with 5% CO₂ for 2 h unless noted otherwise.