Culturing Murine Microglial Cell Lines

C8B4 murine microglial (MG) cell lines expressing the classical MG markers MAC1, F4/80, 2-4G2, Iba1 and TREM2 (but not GFAP) were cultured according to the manufacturer’s protocols (ATCC CRL-2540; Manassas VA) and previously published peer-reviewed work from our lab [12 (link),19 (link),21 (link),28 (link),29 (link)]. MG cell cultures were used at 3 days or 1 wk, and contained approximately 95% MG cells and 5% astroglial/oligodendroglial cells. They were prepared and analyzed according to established methods by the ATCC or our own laboratory [19 (link),26 (link),27 (link),29 (link)].

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Bhattacharjee S., Zhao Y., Dua P., Rogaev E.I, & Lukiw W.J. (2016). microRNA-34a-Mediated Down-Regulation of the Microglial-Enriched Triggering Receptor and Phagocytosis-Sensor TREM2 in Age-Related Macular Degeneration. PLoS ONE, 11(3), e0150211.

Publication 2016

CRL-2540

Astroglial cells Cell lines Gfap Microglial cells Murine Oligodendroglial Trem2

Corresponding Organization : Vavilov Institute of General Genetics

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Variable analysis

independent variables

Cell culture duration (3 days or 1 week)

dependent variables

Expression of classical microglial markers (MAC1, F4/80, 2-4G2, Iba1, TREM2)
Percentage of microglial cells (95%) and astroglial/oligodendroglial cells (5%) in the cell cultures

control variables

Cell culture conditions (following manufacturer's protocols and previously published methods)
Cell line (C8B4 murine microglial cell line)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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